EFFECT OF PROGESTERONE ON ACROSOME REACTION, HYPOOSMOTIC SWELLING TEST, AND DNA STABILITY IN HUMAN SPERMATOZOA
نویسندگان
چکیده
منابع مشابه
Effects of gamma-amino butyric acid, progesterone and oestradiol on human spermatozoa acrosome reaction
The acrosome reaction (AR) is influenced by the action of several molecules such as GABA, P 4 , and E 2 . Objective: to determine the interaction between GABA / E 2 , and GABA / P 4 in the AR. Methods: Washed human spermatozoa capacitated for 2 h were incubated in the following conditions: BWW medium alone (control) or BWW plus: P 4, E 2, GABA, GABA+ P 4 or GABA+ E 2. The % of AR was determined...
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Platelet activating factor (PAF) is a novel signaling phospholipids that in addition to platelet activation has many biological properties. The acrosome reaction, as an essential step in mammalian fertilization, can occur in response to several agents such as PAF. Therefore, the present study aimed to assess the effect of PAF on the motility and acrosome reaction of ram spermatozoa. Semen was c...
متن کاملZona pellucida and progesterone-induced Ca2+ signaling and acrosome reaction in human spermatozoa.
The mammalian acrosome reaction (AR) is the fusion, at multiple points, of the outer acrosomal membrane with the overlying plasma membrane. This fusion causes the release of acrosomal contents and the exposure of the inner acrosomal membrane. The time taken for this process to occur is not accurately known. Estimates for AR in mouse spermatozoa vary from ;2 minutes after stimulation with solubi...
متن کاملEffect of in vitro incubation on spontaneous acrosome reaction in fresh and cryopreserved human spermatozoa.
OBJECTIVES Capacitation and acrosome reaction are prerequisites for fertilization. However, in vitro capacitation is not necessary for an agonist-induced acrosome reaction. We studied whether in vitro capacitation is important in spontaneous acrosome reaction and analyzed how capacitation before cryopreservation influences the acrosomal status of thawed spermatozoa. METHODS Semen specimens fr...
متن کاملRole of Calcium and Calcium Channels in Progesterone Induced Acrosome Reaction in Caprine Spermatozoa
There are several physiological and pharmacological evidences indicating that opening of voltage dependent Ca channels play a critical role in induction of acrosome reaction in mammalian sperm. We determined the intracellular free Ca concentration in ejaculated goat sperm using a fluorescent, Ca-specific probe, Fura2/AM, after the suspension of sperm in KRB medium, capable of sustaining capacit...
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ژورنال
عنوان ژورنال: Archives of Andrology
سال: 1999
ISSN: 0148-5016
DOI: 10.1080/014850199262931